Direct In Situ RT-PCR (Record no. 49413)

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fixed length control field 01828nam a2200313Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625153956.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15232
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245 10 - TITLE STATEMENT
Title Direct In Situ RT-PCR
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Volume/sequential designation Methods in Molecular Biology, 789, p.111-126, 2011
520 3# - SUMMARY, ETC.
Summary, etc. In situ polymerase chain reaction (PCR)is a histological technique that exploits the advantages of PCR for detection of mRNA directly in tissue sections. It somehow conjugates together PCR and in situ hybridization that is more traditionally employed for mRNA localization in cell organelles, intact cells, or tissue sections. This chapter describes the application of in situ PCR for neuropeptide mRNA localization. We provide here a detailed protocol for direct in situ reverse transcription (RT)PCR (RT-PCR)with nonradioactive probes after fixation and paraffin embedding or cryosectioning. Digoxigenin-labeled nucleotides (digoxigenin-11-dUTP)are incorporated in the PCR product after RT and subsequently detected with an anti-digoxigenin antibody conjugated with alkaline phosphatase. The procedure can be modified for use with fluorescent probes and employed in combination with enzyme/fluorescence immunocytochemical labeling.
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Topical term or geographic name entry element IN SITU PCR
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Topical term or geographic name entry element MRNA
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Topical term or geographic name entry element REVERSE TRANSCRIPTION
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Topical term or geographic name entry element DIGOXIGENIN
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Topical term or geographic name entry element IN SITU HYBRIDIZATION
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Topical term or geographic name entry element ALKALINE PHOSPHATASE
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Topical term or geographic name entry element HISTOLOGY
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Topical term or geographic name entry element NEUROPEPTIDES
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Topical term or geographic name entry element CGRP
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lossi, L.
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Personal name Gambino, G.
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Personal name Salio, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Merighi, A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1sucxSYSsJwKu0dg0fI0enDoWKfAS1OsD/view?usp=drivesdk">https://drive.google.com/file/d/1sucxSYSsJwKu0dg0fI0enDoWKfAS1OsD/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15232 25.06.2025 25.06.2025 Documentos solicitados