Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences (Record no. 49518)
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| fixed length control field | 01705nam a2200169Ia 4500 |
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| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160131.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15337 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
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| 245 10 - TITLE STATEMENT | |
| Title | Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Protocols, 2(11), p.2782-2795, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | In this protocol we describe the in situ PCR method for the ampli?cation of both DNA and mRNA targets [in situ reverse transcriptasePCR (RT-PCR)], from frozen or paraf?n-?xed tissue sections, cell culture or other single-cell suspensions. Detection of amplicons can be achieved by the hybridization and detection of labeled probes. The protocol includes the following steps: (i)tissue preparation, (i)in situ PCR (or in situ RT-PCR), (iii)probe hybridization, (iv)signal detection. The technique has high sensitivity (geometrically PCR-amplifying 150-350 bp fragments of a gene of interest in situ)and speci?city (derived from in situ hybridization with speci?c ?uorescent or biotinylated probes for the target genes). The ability to identify individual cells, expressing or carrying specific genes of interest in a latent form in a tissue section under the microscope provides a visual account of silent genes, and allows the determination of various aspects of normal versus pathological conditions, or latent versus active viral replication. An average of 48 h is required to carry out the technique. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bagasra, O. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1DO0n4iD4fr7vgD5lCNMTiU90GyPYK8hk/view?usp=drivesdk">https://drive.google.com/file/d/1DO0n4iD4fr7vgD5lCNMTiU90GyPYK8hk/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15337 | 25.06.2025 | 25.06.2025 | Documentos solicitados |