Stable Incorporation of Plasmid DNA into Higher Plant Cells: the Molecular Basis of Crown Gall Tumorigenesis (Record no. 49528)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02216nam a2200241Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160131.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15347 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Stable Incorporation of Plasmid DNA into Higher Plant Cells: the Molecular Basis of Crown Gall Tumorigenesis |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Cell, 11, p.263-271, 1977 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Evidence is presented that crown gall tumors are caused by the incorporation of part of a virulence plasmid carried by the inciting bacterium, Agro- bacterium tumefaciens. The rate of reassociation of labeled plasmid DNA was slightly accelerated in the presence of tobacco crown gall tumor DNA, but not normal tobacco DNA. Treatment of tumor DNA with DNAase abolished the acceleration. To determine whether all plasmid sequences are represented in tumor DNA, the labeled plasmid DNA was separated into specific fragments after digestion with restriction endonuclease Sma I. Renaturation rates for DNA from bands 1, 2, 7, 8, 9,12 and 14 were not affected by tumor DNA. DNA from band 3 showed a slight rate increase in the presence of tumor DNA, indicating 21-27 copies of 14-18 percent of the DNA sequences in this (doublet)band. The band 3 doublet was separated by elec-trophoresis into bands 3a and 3b. Tumor DNA had little effect on the rate of reassociation of labeled band 3a DNA. Band 3b DNA renatured rapidly in the presence of tumor DNA, and its rate increase indicated that approximately 18 copies of 40 percent of band 3b DNA sequences are present per diploid tumor cell. This amounts to 3.7 x lo6 daltons of foreign genetic information and represents a contribution of 0.0011 percent to the DNA content of the tumor cell. The relationship between this plant tumor and virally induced animal tumor systems is discussed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chilton, M.-D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Drummond, M.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Merlo, D.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sciaky, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Montoya, A.L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gordont, M.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nester, E.W. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1um5JS240M0G4IYYxDYanj8vQRBTOM0c7/view?usp=drivesdk">https://drive.google.com/file/d/1um5JS240M0G4IYYxDYanj8vQRBTOM0c7/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15347 | 25.06.2025 | 25.06.2025 | Documentos solicitados |