Immobilization of pancreatic lipase on chitin and chitosan (Record no. 49636)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02047nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160133.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15455 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Immobilization of pancreatic lipase on chitin and chitosan |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Preparative BioChemistry and Biotechnology, 36(2), p.153-163, 2006 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | In this study, porcine pancreatic lipase (EC 3.1.1.3)was immobilized on chitin and chitosan by adsorption and subsequent crosslinking with glutaraldehyde, which was added before (conjugation)or after (crosslinking)washing unbound proteins. Conjugation proved to be the better method for both supports. The properties of free and immobilized enzymes were also investigated and compared. The results showed that the pH optimum was shifted from 8.5 to 9.0 for both the immobilized enzymes. Also, the optimum temperature was shifted from 30 to 408C for chitinenzyme and to 458C for chitosan-enzyme conjugates. The immobilization ef?ciency is low, but the immobilized enzymes have good reusability and stability (storage and operational). Besides these properties, the immobilized lipases were also suitable for catalyzing esteri?cation reactions of fatty acids and fatty alcohols, both with a medium chain length. According to our results, esteri?cation activities of immobilized lipases were two- and four-fold higher for chitosan- and chitin-enzyme, than for the free enzyme, respectively. The immobilization procedure shows a great potential for commercial applications of the immobilized lipase, a relatively low cost commercial enzyme. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENZYME IMMOBILIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ENZYMATIC ESTERIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHITIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHITOSAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PANCREATIC LIPASE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kilinc, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Teke, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Onal, S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1A3vPAtJRZbOeJq2yrl1aygHI-EZCalMS/view?usp=drivesdk">https://drive.google.com/file/d/1A3vPAtJRZbOeJq2yrl1aygHI-EZCalMS/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15455 | 25.06.2025 | 25.06.2025 | Documentos solicitados |