Effect of Preincubation of Protoplasts on PEG-induced Fusion of Plant Cells . (Record no. 49685)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01527nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160134.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15505 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Effect of Preincubation of Protoplasts on PEG-induced Fusion of Plant Cells . |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Zeitschrift für Pflanzenphysiologie, 79, p.459-464, 1976 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Protoplasts from pea leaves (Pisum sativum)and vetch cell suspension cultures (Vicia hajastana)were fused by agglutination with polyethylene glycol (PEG 1540). The frequency of heterokaryocytes was a function of the duration of preincubation, i.e. the time lapsed between the removal of cell wall digesting enzymes from the isolated protoplasts and agglutination with PEG. The yield of heterokaryocytes decreased from 9 percent after 5 min to 1 percent after 2 h of preincubation. The most obvious drop occurred between 5 and 15 min of preincubation. The decrease in fusion frequency may be due to rapid cell wall regeneration in vetch protoplasts. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTOPLASTS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PEG-INDUCED FUSION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FREQUENCY OF HETEROKARYOCYTES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PISUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | VICIA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Weber, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Constabel, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Williamson, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fowke, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gamborg, O.L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/10IPUn_1jZ2o_u8u9MTXn4oDNajfKwXIP/view?usp=drivesdk">https://drive.google.com/file/d/10IPUn_1jZ2o_u8u9MTXn4oDNajfKwXIP/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15505 | 25.06.2025 | 25.06.2025 | Documentos solicitados |