Designing cross-linked lipase aggregates for optimum performance as biocatalysts (Record no. 49705)

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control field 20250625160134.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15525
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Designing cross-linked lipase aggregates for optimum performance as biocatalysts
490 0# - SERIES STATEMENT
Volume/sequential designation Biocatalysis and Biotransformation, 26(3), p.235-242, 2008
520 3# - SUMMARY, ETC.
Summary, etc. Cross-linked enzyme aggregates (CLEAs)are prepared by precipitation of an enzyme and then chemical cross-linking the precipitate. Three CLEAs of lipase with glutaraldehyde concentrations of 10 mM (CLEA A), 40 mM (CLEA B)and 60 mM (CLEA C)were prepared. Studies show that there is a trade-off between thermal stability vs transesterification/ hydrolysis rate vs enantioselectivity. The initial rates for transesterification of b-citronellol for the uncross-linked enzyme and CLEAs A, B and C were 243, 167, 102 and 40 mmol mg 1 h 1, respectively. Their thermal stabilities in aqueous media, as reflected by their half-life values at 558C, were 6, 9, 13 and 16 h, respectively. The enantioselectivity, E values (for kinetic resolution of b-citronellol by transesterification)were 19, 74, 11 and 6, respectively. These results show that CLEA C was the most thermostable; the uncross-linked enzyme was best at obtaining the highest transesterification rate; and CLEA A was best suited for the enantioselective synthesis. Scanning electron microscopy (SEM)showed that the morphology of CLEA was dependent upon the extent of cross-linking.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CROSS-LINKED ENZYME AGGREGATES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENANTIOSELECTIVITY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FAT SPLITTING, LIPASE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TRANSESTERIFICATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element THERMAL STABILITY
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Majumder, A.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mondal, K.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Singh, T.P.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gupta, M.N.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1T5uIvHVDV5Ij_ws8EDpj5Jj8Vw9mTwvq/view?usp=drivesdk">https://drive.google.com/file/d/1T5uIvHVDV5Ij_ws8EDpj5Jj8Vw9mTwvq/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15525 25.06.2025 25.06.2025 Documentos solicitados