Uptake of Plasmid DNA by Protoplasts from Synchronized Soybean Cell Suspension Cultures (Record no. 49713)

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000 -LEADER
fixed length control field 01375nam a2200229Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160134.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15533
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245 10 - TITLE STATEMENT
Title Uptake of Plasmid DNA by Protoplasts from Synchronized Soybean Cell Suspension Cultures
490 0# - SERIES STATEMENT
Volume/sequential designation Z. Pjlanzenphysiol., 105, p.467-470, 1982
520 3# - SUMMARY, ETC.
Summary, etc. Cell suspension cultur~s of soybean were synchronized by accumulation of cells at the Gil S boundary with 5-fluorodeoxyuridine. Upon removal of fluorodeoxyuridine and addition of thymidine, viable cells resume growth and enter mitosis with increased synchrony. Protoplasts were isolated from cells at varying times following release from inhibition and assayed for uptake of 3H-pBR322 plasmid DNA. Uptake into DNase-resistant, trichloroacetic acid -insoluble form was 2.5 to 4.5-fold higher in S phase than in GI or G2 phase protoplasts. Endo-genous soybean deoxyribonuclease activities did not vary over the cell cycle.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GLYCINE MAX
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA UPTAKE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FLUORODEOXYURIDINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PROTOPLASTS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SYNCHRONIZATION
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cress, D.E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1ei1ruWY0SP0rXSzRLMtImYvYjBeQzQch/view?usp=drivesdk">https://drive.google.com/file/d/1ei1ruWY0SP0rXSzRLMtImYvYjBeQzQch/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15533 25.06.2025 25.06.2025 Documentos solicitados