MARC details
| 000 -LEADER |
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| fixed length control field |
03046nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160135.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-15584 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
In situ detection of Esr proteins secretion during maize microspore embryogenesis and their secretion blockage show effects on the culture progression |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Functional Plant Biology, 37(10), p.985-994, 2010 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
In vitro plant cells in culture release proteins and carbohydrates, but the active molecules responsible for sustaining the switch in embryogenic development and progression have not yet been identified. In maize, the Esr genes encode for small hydrophilic proteins and are expressed in the restricted region of maize endosperm surrounding the embryo, the embryo surrounding region (ESR). In the present work, the possible influence of secreted molecules in the liquid medium during microspore-derived embryo development, and specifically the presence of Esr proteins has been analysed in maize microspore cultures. The study has been carried out by in situ monitoring of the structural and cellu lar organization of developing embryos and the subcellular localization of the Esr proteins by immunofluorescence and immunogold labelling. The results obtained using confocal and electron microscopy revealed that Esrproteins were localized in elements of the secretory pathway and cell walls in microspore-derived embryo cells during early embryogenesis. Esr proteins were also detected in the liquid medium of maize microspore cultures and accumulated at 20 days of culture. Tunicamycin treatment to block protein glycosilation and therefore secretion inhibited microspore-derived embryo development, which was subsequently recovered by supplementation with medium containing all the secreted factors from a well-developed microspore culture. Esr labelling was not present in non-developing microspore embryos of cultures treated with tunicamycin, whereas labelling was present again in the Golgi elements and secretory vesicles of embryo cells when development was restored. The results indicate that Esr proteins are part of the secreted proteins, which show a nursing or signalling role during in vitro embryo development in maize microspore embryogenesis cultures, and provide new evidence for an endosperm-like function of microspore-derived embryo structures during the early stages. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MAIZE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MICROSPORE EMBRYOGENESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ESR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SIGNALLING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SECRETION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT PEPTIDES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
EMBRYO DEVELOPMENT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ENDOSPERM SURROUNDING REGION. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Testillano, P.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Coronado, M.-J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Matthys-Rochon, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Thierry, A.-M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Risueño, M.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1onumXf3sVJpgYs8DE2dJUiJtowj2zmIc/view?usp=drivesdk">https://drive.google.com/file/d/1onumXf3sVJpgYs8DE2dJUiJtowj2zmIc/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
