Purification and characterization of a common soil component which inhibits the polymerase chain reaction (Record no. 49766)

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fixed length control field 02180nam a2200241Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160135.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15586
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245 10 - TITLE STATEMENT
Title Purification and characterization of a common soil component which inhibits the polymerase chain reaction
490 0# - SERIES STATEMENT
Volume/sequential designation Canadian Journal MicroBiology, 46(7), p.633-642, 2000
520 3# - SUMMARY, ETC.
Summary, etc. DNA prepared from soil usually contains a brown-tinted inhibitor of the polymerase chain reaction (PCR)which limits the sensitivity of this technique for specific detection of microorganisms. To localize the inhibitor, soil fractions were tested for their inhibitory effect on the PCR reaction. A highly inhibitory activity, sufficient to account for the inhibition typically exhibited by soil DNA, was found to be tightly associated with the soil microorganism fraction. After cell breakage, the inhibitory material became soluble, and was not separable from DNA by standard purification procedures. A method was derived by which most of the inhibitory material could be selectively solubilized from the microorganism fraction without cell breakage, using successive washes with buffers differing in EDTA con centration. This technique was used to isolate a substance with characteristics suggesting that it is the major PCR in hibitor contaminating DNA purified from soil. It was found to be an organic, water-soluble compound of high molecular weight, and was present in a variety of soil types from different locations. It was found to be distinctly different in its solubility properties from humic and fulvic acids, and also in its FT-IR and NMR spectra. It forms a complex with protein and may inhibit the PCR reaction by an interaction with Taq DNA polymerase.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FULVIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element HUMIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCR INHIBITOR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOIL DNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOIL MICROORGANISMS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Watson, R.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Blackwell, B.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1jrAm1CUfsw2kWELv6baiRVMkOMlpDOf_/view?usp=drivesdk">https://drive.google.com/file/d/1jrAm1CUfsw2kWELv6baiRVMkOMlpDOf_/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15586 25.06.2025 25.06.2025 Documentos solicitados