A Simple and Powerful Approach for Isolation of Arabidopsis Mutants with Increased Tolerance to H2O2 - Induced Cell Death (Record no. 49771)

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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15591
245 10 - TITLE STATEMENT
Title A Simple and Powerful Approach for Isolation of Arabidopsis Mutants with Increased Tolerance to H2O2 - Induced Cell Death
490 0# - SERIES STATEMENT
Volume/sequential designation Methods in Enzymology, 517, p.203-220, 2013
520 3# - SUMMARY, ETC.
Summary, etc. A genetic approach is described to isolate mutants more tolerant to oxidative stress. A collection of T-DNA activation tag Arabidopsis thaliana mutant lines was screened for survivors under conditions that trigger H2O2 -induced cell death. Oxidative stress was induced by applying the catalase (CAT)inhibitor aminotriazole (AT)in the growth media, which results in decrease in CAT enzyme activity, H2O2 accumulation, and subsequent plant death. One mutant was recovered from the screening and named oxr1(oxidative stress resistant 1). The location of the T-DNA insertion was identified by TAILPCR. Oxr1 exhibited lack of cell death symptoms and more fresh weight and chlorophyll content compared to wild type. The lack of cell death correlated with more prominent 2 induction of anthocyanins synthesis in oxr1. These results demonstrate the feasibility of AT as a screening agent for the isolation of oxidative stress-tolerant mutants and indicate a possible protective role for anthocyanins against AT-induced cell death. The chapter includes protocols for ethyl methanesulfonate mutagenesis, mutant screening using AT, T-DNA identification by TAIL-PCR, CAT activity measurements, and determination of malondialdehyde, chlorophyll, and anthocyanins.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Gechev, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hille, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mehterov, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Denev, I.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1pMNwuBLtrI4_QTA3ajkMOr0YiwwatBdq/view?usp=drivesdk">https://drive.google.com/file/d/1pMNwuBLtrI4_QTA3ajkMOr0YiwwatBdq/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15591 25.06.2025 25.06.2025 Documentos solicitados