Using Hyper as a Molecular Probe to Visualize Hydrogen Peroxide in Living Plant Cells: A Method with Virtually Unlimited Potential in Plant Biology (Record no. 49775)
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| fixed length control field | 02079nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251013162658.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15595 |
| 245 10 - TITLE STATEMENT | |
| Title | Using Hyper as a Molecular Probe to Visualize Hydrogen Peroxide in Living Plant Cells: A Method with Virtually Unlimited Potential in Plant Biology |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Methods in Enzymology, 527, p.275-290, 2013 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Reactive oxygen species (ROS)are highly reactive reduced oxygen molecules that play a myriad of roles in animal and plant cells. In plant cells, the production of ROS occurs as a result of aerobic metabolism during respiration and photosynthesis. Therefore mitochondria, chloroplasts, and peroxisomes constitute an important source of ROS. However, they can be produced in response to many physiological stimuli such as pathogen attack, hormone signaling, abiotic stresses, or during cell wall organization and plant morphogenesis. Monitoring ROS in plant cells has been limited to biochemical assays and use of fluorescent probes, however, the irreversible oxidation of the fluorescent dyes make it impossible to visualize dynamic changes of ROS. Hyper is a recently developed live cell probe for H2O2 and consists of a circularly permutated YFP (cpYFP)inserted into the regulatory domain of the Escherichia coli hydrogen peroxide (H2O2)sensor OxyR rendering it a H2O2 specific ratiometric, and therefore quantitative probe. Herein, we describe a protocol for using Hyper as a dynamic probe for H2O2 in Arabidopsis with virtually unlimited potential to detect H2O2 throughout the plant and under a broad range of developmental and environmental conditions. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hernández-Barrera, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Quinto, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Johnson, E.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wu, H.-M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cheung, A.Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cárdenas, L. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1Hex-5scpWG7RPTAzddO3QDdTzLiOE5kM/view?usp=drivesdk">https://drive.google.com/file/d/1Hex-5scpWG7RPTAzddO3QDdTzLiOE5kM/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15595 | 25.06.2025 | 25.06.2025 | Documentos solicitados |