Plant Regeneration by Somatic Embryogenesis From Parts of Cultured Mature Embryos of Pennisetum americanum (L.)K. Schum. (Record no. 49863)

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control field 20250625160137.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15683
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245 10 - TITLE STATEMENT
Title Plant Regeneration by Somatic Embryogenesis From Parts of Cultured Mature Embryos of Pennisetum americanum (L.)K. Schum.
490 0# - SERIES STATEMENT
Volume/sequential designation Z. Pflanzenphysiol., 111(4), p.319-325, 1983
520 3# - SUMMARY, ETC.
Summary, etc. Whole and excised parts of mature embryos and seedlings of Pennisetum americanum (L.)K. Schum. were cultured in Murashige and Skoog's medium (MS)supplemented with 2,4-dichlorophenoxyacetic acid and coconut milk. A soft friable callus, which later developed only roots, was formed when whole embryos or isolated mesocotyl and radicle were cultured. A compact embryogenic callus was induced only from the shoot apex and the two youngest leaf primordia excised from mature embryos or 4-5 day old seedlings. The complete removal of mesocotyl tissue from the base of the shoot apex at the time of culture was critical for the formation of compact callus, and for inhibiting the development of the friable callus. The embryoids developed by a sequence of internal segmenting divisions from single embryogenic cells, and showed a well organized embryonic axis with a closed vascular system, a coleoptile, a coleorhiza, and a scutellum. Upon transfer to media which promoted shoot and root growth, normal plantlets were formed and transferred successfully to soil.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PENNISETUM AMERICANUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CEREALS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GRASSES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE CULTURE.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Botti, C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vasil, I. K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/15ZySp5Es--UBLmYjw-63KzYEIgY5PIp6/view?usp=drivesdk">https://drive.google.com/file/d/15ZySp5Es--UBLmYjw-63KzYEIgY5PIp6/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15683 25.06.2025 25.06.2025 Documentos solicitados