Application of an integrated LC-UV-MS-NMR platform to the identification of secondary metabolites from cell cultures: benzophenanthridine alkaloids from elicited Eschscholzia californica(california poppy)cell cultures (Record no. 49874)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02148nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160137.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15695 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Application of an integrated LC-UV-MS-NMR platform to the identification of secondary metabolites from cell cultures: benzophenanthridine alkaloids from elicited Eschscholzia californica(california poppy)cell cultures |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Analitycal Methods, 4(5), p.1315-1325, 2012 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Plant cell and tissue cultures are a scalable and controllable alternative to whole plants for obtaining natural products of medical relevance. Cultures can be optimized for high yields of desired metabolites using rapid profiling assays such as HPLC. We describe an approach to establishing a rapid assay for profiling cell culture expression systems using a novel microscale LC-UV-MS-NMR platform, designed to acquire both MS and NMR each at their optimal sensitivity, by using nanosplitter MS from 4 mm analytical HPLC columns, and offline microdroplet NMR. The approach is demonstrated in the analysis of elicited Eschscholzia californicacell cultures induced with purified yeast extract to produce benzophenanthridine alkaloids. Preliminary HPLC-UV provides an overview of the changes in the production of alkaloids with time after elicitation. At the time point orresponding to the production of the most alkaloids, the integrated LC-MS-microcoil NMR platform is used for structural identification of extracted alkaloids. Eight benzophenanthridine alkaloids were identified at the sub- icrogram level. This paper demonstrates the utility of the nanosplitter LC-MS/microdroplet NMR platform when establishing cell culture expression systems. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gathungu, Rose M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Oldham, John T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bird, Susan S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee-Parsons, Carolyn W. T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vouros, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kautz, R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1i-VQwc6J7MuQWuZ6vtWG8btpD9xZJ457/view?usp=drivesdk">https://drive.google.com/file/d/1i-VQwc6J7MuQWuZ6vtWG8btpD9xZJ457/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15695 | 25.06.2025 | 25.06.2025 | Documentos solicitados |