Separation and characterization of rice proteins (Record no. 49887)

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fixed length control field 01693nam a2200229Ia 4500
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control field MX-MdCICY
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control field 20250625160137.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15708
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Title Separation and characterization of rice proteins
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Volume/sequential designation Electrophoresis, 15, p.708-720, 1994
520 3# - SUMMARY, ETC.
Summary, etc. Rice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus)were isolated and then separated by two-dimensional gel electrophoresis (2-DE). The protein spots were characterized according to molecular weight, isoelectric point and partial amino-terminal sequence. Electrophoresis was carried out by isoelectric focusing (IEF), nonequilibrium pH gradient electrophoresis (NEPHGE)and immobilized pH gradient (IPG)in the first dimension, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)in the second dimension. With the aid of nine marker proteins, the patterns of IEF, NEPHGE and IPG 2-DE gels were graphically combined by computer into a single synthetic image for each tissue, respectively, and these images for the nine tissues and one organelle were again combined into a single 2-DE image for the integrated rice protein spots. The rice 2-DE gel image resolved 4892 proteins. About 3
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tsugita, A.
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Personal name Kawakami, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Uchiyama, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kamo, M.
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Personal name Miyatake, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nozu, N.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1Hw_dedTqIhWcC637mliYwKLGwtgqxMZ1/view?usp=drivesdk">https://drive.google.com/file/d/1Hw_dedTqIhWcC637mliYwKLGwtgqxMZ1/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15708 25.06.2025 25.06.2025 Documentos solicitados