Low external ph prevents cell elongation but not multiplication of embryogenic carrot cells (Record no. 49988)

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control field 20250625160139.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15811
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245 10 - TITLE STATEMENT
Title Low external ph prevents cell elongation but not multiplication of embryogenic carrot cells
490 0# - SERIES STATEMENT
Volume/sequential designation Physiologia Plantarum, 84(4), p.495-501, 1992
520 3# - SUMMARY, ETC.
Summary, etc. Embryogenic cultures of cultivated carrot (Daucus carota cv. Scarlet Nantes)were initiated from seedling hypocotyls on hormone-containing nutrient medium and from wounded zygotic embryos on hormone-free medium. Both of these cultures were maintained with continuous multiplication as unorganized, embryogenic cell masses on hormone-free medium at pH 4.0, containing NH4+ as the sole nitrogen source. When grown on hormone-free medium at pH 4.0, neither culture contained any elongated cells. Virtually all cells were densely cytoplasmic and nearly spherical. Some cells were enlarged, not densely cytoplasmic, but always spherical. When either culture was transferred to an auxin-containing medium at pH 5.8, numerous elongated cells were produced. Elongated cells were observed when either naphthalene-acetic acid or 2,4-dichlorophenoxyacetic acid was used, and whether the nitrogen source was NH4+ alone or a combination of NH4+ and NO3-. Elongated cells were more abundant when a combined nitrogen source was used. When cultures containing elongated cells were transferred to and multiplied on hormone-free or hormone-containing medium buffered at pH 4.0, all elongated cells disappeared after 2 weeks. No elongated cells were observed in any of the lines tested at pH 4.0. These results clearly show that it was the pH of the culture medium and not the presence or absence of an auxin or the nitrogen source(s)that permitted or prevented cell elongation in the embryogenic cultures tested.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AUXIN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL ELONGATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DAUCUS CAROTA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PH
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE CULTURE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Smith, Dl
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Krikorian, Ad
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1EzZUZSvSizFKxqyGLZWx1rQ92C20xO0_/view?usp=drivesdk">https://drive.google.com/file/d/1EzZUZSvSizFKxqyGLZWx1rQ92C20xO0_/view?usp=drivesdk</a>
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