High-resolution insertion-site analysis by linear amplification-mediated PCR (LAM-PCR) (Record no. 50100)
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| 000 -LEADER | |
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| fixed length control field | 02564nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160141.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15924 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | High-resolution insertion-site analysis by linear amplification-mediated PCR (LAM-PCR) |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Nature Methods, 4(5), p.1051-1057, 2007 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Integrating vector systems used in clinical gene therapy have proven their therapeutic potential in the long-term correction of immunodeficiencies. The integration loci of such vectors in the cellular genome represent a molecular marker unique for each transduced cell and its clonal progeny. To gain insight into the physiology of gene-modified hematopoietic repopulation and vector-related influences on clonal contributions, we have previously introduced a technology-linear amplification-mediated (LAM)PCR-for detecting and sequencing unknown DNA flanking sequences down to the single cell level (Supplementary Note online). LAM-PCR analyses have enabled qualitative and quantitative measurements of the clonal kinetics of hematopoietic regeneration in gene transfer studies, and uncovered the clonal derivation of non-leukemogenic and leukemogenic insertional side effects in preclinical and clinical gene therapy studies. The reliability and robustness of this method results from the initial preamplification of the vector-genome junctions preceding nontarget DNA removal via magnetic selection. Subsequent steps are carried out on a semisolid streptavidin phase, including synthesis of double complementary strands, restriction digest, ligation of a linker cassette onto the genomic end of the fragment and exponential PCR(s)with vector- and linker cassette-specific primers. LAM-PCR can be adjusted to all unknown DNA sequences adjacent to a known DNA sequence. Here we describe the use of LAM-PCR analyses to identify 5' long terminal repeat (LTR)retroviral vector adjacent genomic sequences (Fig. 1 and Box 1). |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MOLECULAR MARKER |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RETROVIRUS VECTOR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | STREPTAVIDIN |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schmidt, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Schwarzwaelder, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bartholomae, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zaoui, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ball, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pilz, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Braun, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Braun, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Von Kalle, C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1YR75gpkSKlqOKg61v6GhkdcVpVxyjIqH/view?usp=drivesdk">https://drive.google.com/file/d/1YR75gpkSKlqOKg61v6GhkdcVpVxyjIqH/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15924 | 25.06.2025 | 25.06.2025 | Documentos solicitados |