Synergistic Activity of the Plant Defensin HsAFP1 and Caspofungin against Candida albicans Biofilms and Planktonic Cultures. (Record no. 50124)
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| fixed length control field | 02970nam a2200337Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160142.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15948 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Synergistic Activity of the Plant Defensin HsAFP1 and Caspofungin against Candida albicans Biofilms and Planktonic Cultures. |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | PLoS One, 10(8), p.1-22, 2016 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Plant defensins are small, cysteine-rich peptides with antifungal activity against a broad range of yeast and fungi. In this study we investigated the antibiofilm activity of a plant defensin from coral bells (Heuchera sanguinea), i.e. HsAFP1. To this end, HsAFP1 was heterologously produced using Pichia pastoris as a host. The recombinant peptide rHsAFP1 showed a similar antifungal activity against the plant pathogen Fusarium culmorum as native HsAFP1 purified from seeds. NMR analysis revealed that rHsAFP1 consists of an ?-helix and a triple-stranded antiparallel ?-sheet stabilised by four intramolecular disulfide bonds. We found that rHsAFP1 can inhibit growth of the human pathogen Candida albicans as well as prevent C. albicans biofilm formation with a BIC50 (i.e. the minimum rHsAFP1 concentration required to inhibit biofilm formation by 50 per cent as compared to control treatment)of 11.00 ± 1.70 ?M. As such, this is the first report of a plant defensin exhibiting inhibitory activity against fungal biofilms. We further analysed the potential of rHsAFP1 to increase the activity of the conventional antimycotics caspofungin and amphotericin B towards C. albicans. Synergistic effects were observed between rHsAFP1 and these compounds against both planktonic C. albicans cells and biofilms. Most notably, concentrations of rHsAFP1 as low as 0.53 ?M resulted in a synergistic activity with caspofungin against pre-grown C. albicans biofilms. rHsAFP1 was found non-toxic towards human HepG2 cells up to 40 ?M, thereby supporting the lack of a general cytotoxic activity as previously reported for HsAFP1. A structure-function study with 24-mer synthetic peptides spanning the entire HsAFP1 sequence revealed the importance of the ?-core and its adjacent regions for HsAFP1 antibiofilm activity. These findings point towards broad applications of rHsAFP1 and its derivatives in the field of antifungal and antibiofilm drug development. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CANDIDA ALBICANS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIOFILMS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vriens, K |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cools, Tl |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Harvey, Pj |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Craik, Dj |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Spincemaille, P |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cassiman, D |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Braem, A |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Braem, A |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nibbering, Ph |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Drijfhout, Jw |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | De Coninck, B |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cammue, Bp |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thevissen K |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/14mNxAwcJ_3P_XviJV6FcbyDZV7TAaSgW/view?usp=drivesdk">https://drive.google.com/file/d/14mNxAwcJ_3P_XviJV6FcbyDZV7TAaSgW/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15948 | 25.06.2025 | 25.06.2025 | Documentos solicitados |