Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique (Record no. 50128)
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| fixed length control field | 02902nam a2200313Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160142.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-15952 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | BioMaterials, 29(7), p.825-833, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | There is a clinical need for a tissue-engineered vascular graft (TEVG), and combining stem cells with biodegradable tubular scaffolds appears to be a promising approach. The goal of this study was to characterize the incorporation of muscle-derived stem cells (MDSCs)within tubular poly(ester urethane)urea (PEUU)scaffolds in vitro to understand their interaction, and to evaluate the mechanical properties of the constructs for vascular applications. Porous PEUU scaffolds were seeded with MDSCs using our recently described rotational vacuum seeding device, and cultured inside a spinner flask for 3 or 7 days. Cell viability, number, distribution and phenotype were assessed along with the suture retention strength and uniaxial mechanical behavior of the TEVGs. The seeding device allowed rapid even distribution of cells within the scaffolds. After 3 days, the constructs appeared completely populated with cells that were spread within the polymer. Cells underwent a population doubling of 2.1-fold, with a population doubling time of 35 h. Stem cell antigen-1 (Sca-1)expression by the cells remained high after 7 days in culture (77±20 per cent vs. 66±6 per cent at day 0)while CD34 expression was reduced (19±12 per cent vs. 61±10 per cent at day 0)and myosin heavy chain expression was scarce (not quantified). The estimated burst strength of the TEVG constructs was 2127±900 mmHg and suture retention strength was 1.3±0.3 N. We conclude from this study that MDSCs can be rapidly seeded within porous biodegradable tubular scaffolds while maintaining cell viability and high proliferation rates and without losing stem cell phenotype for up to 7 days of in-vitro culture. The successful integration of these steps is thought necessary to provide rapid availability of TEVGs, which is essential for clinical translation. © 2007 Elsevier Ltd. All rights reserved. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | BIODEGRADABLE POLYMERS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CELL CULTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MECHANICAL PROPERTIES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | POLYESTERS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | SCAFFOLDS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | TISSUE ENGINEERING |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nieponice, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soletti, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Guan, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Deasy, B.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Huard, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wagner, W.R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vorp, D.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1yhsWXpPc4YNexmTy2XYJ1dlr7snShXPm/view?usp=drivesdk">https://drive.google.com/file/d/1yhsWXpPc4YNexmTy2XYJ1dlr7snShXPm/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-15952 | 25.06.2025 | 25.06.2025 | Documentos solicitados |