Defense Gene Expression Associated with Biotrophic Phase of Mycosphaerella fijiensis M. Morelet Infection in Banana (Record no. 50172)

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fixed length control field 02360nam a2200241Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160143.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-15996
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Defense Gene Expression Associated with Biotrophic Phase of Mycosphaerella fijiensis M. Morelet Infection in Banana
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Disease, 100(6), p.1170-1175, 2016
520 3# - SUMMARY, ETC.
Summary, etc. Banana black leaf streak, caused by Mycosphaerella fijiensis M. Morelet, is a primary phytosanitary concern in export of this fruit around the world. To develop improved cultivars resistant to this disease, an understanding of host response to infection is necessary. In this study, we obtained expression data on 14,872 genes by microarray analysis in the resistant genotype Musa acuminata subsp. burmannicoides 'Calcutta 4' after inoculation with Mycosphaerella fijiensis. From these data, 16 genes were analyzed as potential reference genes and 12 genes were identified as potential early indicators of the onset of the host defense response. Subsequently, these genes were analyzed by quantitative reverse-transcription polymerase chain reaction in susceptible 'Williams' and resistant Calcutta 4. The 18S and 26S ribosomal subunit genes in both cultivars showed the best characteristics as reference genes. In all, 5 of the 12 defense genes expressed shortly after infection (peroxidase, pathogenesis-related [PR]-4, PR-10, phenylalanine ammonia-liase, and disease resistance response 1)showed overexpression in Calcutta 4 between 6 and 24 h after inoculation as opposed to Williams, which did not show overexpression after 144 h. Early induction of defense-related genes could be a key component of the resistance of the Calcutta 4 genotype against M. fijiensis. In addition, these five genes could be used as indicators of the activation of defense responses in the interaction between banana and M. fijiensis.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MYCOSPHAERELLA FIJIENSIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BANANO
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rodriguez, Hector A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Rodriguez-Arango, Esperanza
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Morales, Juan G.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kema, Gert
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arango, Rafael E.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/11eVgemoc1_2GY9fQyriiZc90OrVmY4UM/view?usp=drivesdk">https://drive.google.com/file/d/11eVgemoc1_2GY9fQyriiZc90OrVmY4UM/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-15996 25.06.2025 25.06.2025 Documentos solicitados