Two DNA antirestriction systems of bacteriophage P1, darA, and darB: characterization of darA- phages (Record no. 50184)

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fixed length control field 01740nam a2200241Ia 4500
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control field MX-MdCICY
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control field 20250625160143.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16008
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Title Two DNA antirestriction systems of bacteriophage P1, darA, and darB: characterization of darA- phages
490 0# - SERIES STATEMENT
Volume/sequential designation Virology, 157(1), p.156-66, 1987
520 3# - SUMMARY, ETC.
Summary, etc. Bacteriophage P1 is only weakly restricted when it infects cells carrying type I restriction and modification systems even though DNA purified from P1 phage particles is a good substrate for type I restriction enzymes in vitro. Here we show that this protection against restriction is due to the products of two phage genes which we call darA and darB (dar for defense against restriction). Each of the dar gene products provides protection against a different subset of type I restriction systems. The darA and darB gene products are found in the phage head and protect any DNA packaged into a phage head, including transduced chromosomal markers, from restriction. The proteins must, therefore, be injected into recipient cells along with the DNA. The proteins act strictly in cis. For example, upon double infection of restricting cells with dar+ and dar- P1 phages, the dar+ genomes are protected from restriction while the dar- genomes are efficiently restricted.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BACTERIOPHAGE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NONHUMAN
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Topical term or geographic name entry element PRIORITY JOURNAL
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Iida S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Streiff Mb
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Bickle Ta
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Arber W.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1FzAeTLoZAdq08q2x9LfvZXdGD_MT52dV/view?usp=drivesdk">https://drive.google.com/file/d/1FzAeTLoZAdq08q2x9LfvZXdGD_MT52dV/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16008 25.06.2025 25.06.2025 Documentos solicitados