A fluorescent method for the rapid staining and quantitation of proteins in sodium dodecyl sulfate-polyacrylamide gels (Record no. 50240)
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| 000 -LEADER | |
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| fixed length control field | 01712nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160144.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16065 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A fluorescent method for the rapid staining and quantitation of proteins in sodium dodecyl sulfate-polyacrylamide gels |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Electrophoresis, 6(11), p.527-531, 1985 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Fluorescence studies carried out in solution indicate that in the presence of the anionic detergent sodium dodecyl sulfate (SDS)proteins are able to bind the noncovalent dye 1-anilinonaphthalene-8-sulfonate (ANS). The interaction of this hydrophobic dye with protein-SDS complexes is probably responsible for the fluorescence of protein bands observed by UV-transillumination of polyacrylamide gels stained with ANS. It is shown that the staining of SDS-polyacrylamide gels with ANS after electrophoresis allows the detection and quantitative estimation of proteins and peptides having different properties. The electrophoretic patterns obtained using ANS are equivalent to those obtained using Coomassie Brilliant Blue R-250. The fluorescent staining method described in this work is simpler and much more rapid than conventional methods using visible dyes. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | FLUORESCENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | METHODOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | NONHUMAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | PROTEIN STAINING |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aragay, A.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Diaz, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Daban, J.R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/15ilaL93UabPnZ_Jy-_LBI0e8GYXE3cfE/view?usp=drivesdk">https://drive.google.com/file/d/15ilaL93UabPnZ_Jy-_LBI0e8GYXE3cfE/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16065 | 25.06.2025 | 25.06.2025 | Documentos solicitados |