MARC details
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| fixed length control field |
02326nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160144.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16068 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Sodium oxalate corrects calcium interference in lowry protein assay |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Journal of Surgical Research, 47(3), p.273-275, 1989 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
We have previously noted that high levels (10 mM)of calcium interfere with protein determination in the Lowry assay. While there are numerous reports in the literature of substances interfering with the Lowry assay, none of these interfering substances are as ubiquitous as calcium. We hypothesizied that physiologic levels of calcium may interfere with the protein determination as measured by the Lowry assay. To test this hypothesis, bovine serum albumin standards from 12.5 to 1600 ?g/ml were tested in the Lowry assay in the presence of calcium chloride concentrations from 0 to 5 mM. In low protein concentrations (12.5-50 ?g/ml)1 to 2 mM calcium induced errors of up to 384 percent. In samples containing 100 to 400 ?g/ml protein, 1 to 2 mM calcium induced errors of up to 40 percent . The addition of sodium oxalate to calcium-spiked protein samples significantly reduced the errors in protein determination at all calcium concentrations tested. This effect also is seen throughout the range of protein concentrations tested (50-1600 ?g/ml). In most cases sodium oxalate pretreatment of samples used in the Lowry assay reduces errors by 70-95 percent . Physiologic levels of calcium induce significant errors in the estimation of protein concentration using the Lowry method. Routine pretreatment of Lowry samples with sodium oxalate will provide a more accurate estimation of the protein concentration in biological systems. © 1989. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANIMAL CELL |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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ASSAY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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CATTLE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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DIAGNOSIS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
LOWRY PROTEIN ASSAY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
METHODOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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NONHUMAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
OXALATE SODIUM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PRIORITY JOURNAL |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Morrissey, T.B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Woltering, E.A. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1wIg9CNRiXAn_ZSQhR3hnJiBukKuZ4AfP/view?usp=drivesdk">https://drive.google.com/file/d/1wIg9CNRiXAn_ZSQhR3hnJiBukKuZ4AfP/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
