Isoelectric focusing and two-dimensional analysis of purified nitrate reductase from Aspergillus nidulans (Record no. 50292)

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fixed length control field 02342nam a2200253Ia 4500
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control field MX-MdCICY
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control field 20250625160145.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16117
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Title Isoelectric focusing and two-dimensional analysis of purified nitrate reductase from Aspergillus nidulans
490 0# - SERIES STATEMENT
Volume/sequential designation Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 706(2), 7, p.203-211, 1982
520 3# - SUMMARY, ETC.
Summary, etc. The assimilatory NADPH-nitrate oxidoreductase (EC 1.6.6.3)from Aspergillus nidulans was purified by means of affinity chromatography and analyzed by agarose isoelectric focusing and two-dimensional electrophoresis. NADPH-nitrate reductase activity was not activated by oxidation with potassium ferricyanide and was irreversibly inhibited by acrylamide. Electrophoresis of nitrate reductase in 7 percent polyacrylamide gels resulted in rapid loss of enzyme activity. Isoelectric focusing of purified enzyme in agarose gels resulted in one homogeneous band that exhibited NADPH-nitrate reductase, NADPH-cytochrome c reductase and reduced methyl viologen-nitrate reductase activities, which corresponded to an isoelectric point of 6.12 ± 0.05 at 22°C. Two-dimensional electrophoresis of focused nitrate reductase on SDS-polyacrylanide gel slabs yielded a single subunit of 54000 molecular weight. Acid treatment of the enzyme and subsequent isoelectric focusing resulted in a protein with a strongly acidic isoelectric point and reduced methyl viologen-nitrate reductase activity. It released another protein with a strongly basic isoelectric point which was inactive. It is postulated that the overall association of fiavoprotein protomers with both heme and cytochrome b1 components confers a small net negative charge upon the native heteromultimer and accounts for its slightly acidic isoelectric point. © 1982.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ASPERGILLUS NIDULANS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ELECTROPHORESIS, POLYACRYLAMIDE GEL
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ISOELECTRIC FOCUSING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element KINETICS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MOLECULAR WEIGHT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NITRATE REDUCTASES
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Steiner, F.X.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Downey, R.J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/149u9PKXKF81PGGF0Q0d9gef3WnqeEgDp/view?usp=drivesdk">https://drive.google.com/file/d/149u9PKXKF81PGGF0Q0d9gef3WnqeEgDp/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16117 25.06.2025 25.06.2025 Documentos solicitados