Method for Simultaneous Determination of Glutamine Synthetase and Adenosine Triphosphatase Activities on Polyacrylamide Gel (Record no. 50293)
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| fixed length control field | 01767nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160145.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16118 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Method for Simultaneous Determination of Glutamine Synthetase and Adenosine Triphosphatase Activities on Polyacrylamide Gel |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Biochemie und Physiologie der Pflanzen, 178(4), p.243-248, 1983 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | A method for the simultaneous localization of adenosine triphosphatase and glutamine synthetase after non-denaturing polyacrylamide gel electrophoresis is described. The determination of enzyme aetivities is based on the staining for the inorganic phosphate produced by aetivities of both glutamine synthetase and adenosine triphosphatase enzymes. The speeificity of the glutamine synthetase reaction was demonstrated by methionine sulphoximine (MSO)inhibition of the enzyme reaction. Mg2+ -ATPase, Ca2+ -ATPase and glutamine synthetase enzymes were sneeesfully separated on a continuous linear (3-10 percent)polyacrylamide gradient. Glutamine synthetase and ATPase enzymes isolated from Azotobacter bejerinckii and from the green alga Chlamydomonas reinhardii showed different isoenzyme pattern after electrophoretic annlysis. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GLUTAMINE SYNTHETASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ADENOSINE TRIPHOSPHATASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ACTIVITY IN GELS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHLAMYDOMONAS REINHARDII |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AZOTOBACTER BEJERINCKII |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nagy, A.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Csanadi, J |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Turtoczky, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gyurján, I. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1lBIvYzs94xp0WEJWSo5kXY-zXRFc30is/view?usp=drivesdk">https://drive.google.com/file/d/1lBIvYzs94xp0WEJWSo5kXY-zXRFc30is/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16118 | 25.06.2025 | 25.06.2025 | Documentos solicitados |