Gel electrofocusing in tubes containing Sephadex (Record no. 50294)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01903nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160145.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16119 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Gel electrofocusing in tubes containing Sephadex |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Electrophoresis, 5(6), p.343-348, 1984 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Gel electrofocusing (EF)on Sephadex G-75, which provides relatively non-restrictive pore sizes and relatively broad protein concentration distributions (which reduces concentration-dependent aggregation), was adapted for use in glass tubes. This allows for EF with meticulously controlled low temperatures in conjunction with large sample and gel volumes. Specifically, the technique was developed for EF of steroid receptors, which are highly sensitive to temperature (temperature transformed), and whose aggregation behavior excluded the use of polyacrylamide or agarose gels. The technique uses the temperature-controlled apparatus designed for quantitative polyacrylamide gel electrophoresis, with three additions. These are a)a 12- place evacuation device for forming simultaneously 12 cylindrical Sephadex gels (either 6 or 18 mm diameter), b)an electrode arrangement capable of maintaining the same regulated voltage across several gel tubes, and c)a jacket and lid of polyurethane foam to insulate the electrolyte chambers of the EF apparatus. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ELECTROFOCUSING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lan, Bad (Lan, Bad) |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Benor, S (Benor, S) |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Allenmark, S (Allenmark, S) |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sullivan, Jv (Sullivan, Jv) |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fitze, P (Fitze, P) |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jackiw, A (Jackiw, A) |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1A2Lx1QwaG5ridnNg41rFVEN5temYYQ9m/view?usp=drivesdk">https://drive.google.com/file/d/1A2Lx1QwaG5ridnNg41rFVEN5temYYQ9m/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16119 | 25.06.2025 | 25.06.2025 | Documentos solicitados |