Isolation of a carotenoid biosynthesis gene coding for ?-carotene desaturase from Anabaena PCC 7120 by heterologous complementation (Record no. 50322)

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control field MX-MdCICY
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control field 20250625160146.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16148
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Title Isolation of a carotenoid biosynthesis gene coding for ?-carotene desaturase from Anabaena PCC 7120 by heterologous complementation
490 0# - SERIES STATEMENT
Volume/sequential designation FEMS MicroBiology Letters, 106(1), p.99-103, 1993
520 3# - SUMMARY, ETC.
Summary, etc. A screening procedure for carotenoid genes involving heterologous complementation with two different plasmid constructs was developed. The plasmids contained the crtE and crtB genes from Erwinia unredovora together with the phytoene desaturase gene from either Rhodobacter capsulatus or Synechococcus PCC 7942. Transformation in E. coli led to the accumulation of neurosporene and ?-carotene, respectively. Co-transformation with an Anabaena plasmid library resulted in the isolation of the two plasmids, pZDS1 and pZDS1. Their gene products showed the ability to convert neurosporene and ?-carotene into lycopene. In contrast, accumulated phytoene could not be converted. We conclude that the cloned gene codes for the carotenoid biosynthesis gene ?-carotene desaturase (zds).
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAROTENOID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element UNCLASSIFIED DRUG
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ZETA CAROTENE DESATURASE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Linden, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vioque, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sandmann, G.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1lsxgn8NqU1ZLejrC2BnNHQintQMDgNQM/view?usp=drivesdk">https://drive.google.com/file/d/1lsxgn8NqU1ZLejrC2BnNHQintQMDgNQM/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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