MARC details
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03224nam a2200433Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20250625160146.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16159 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Prequels to Synthetic Biology: From Candidate Gene Identification and Validation to Enzyme Subcellular Localization in Plant and Yeast Cells |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Methods in Enzymology, p.167-206, 2016 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Natural compounds extracted from microorganisms or plants constitute an inexhaustible source of valuable molecules whose supply can be potentially challenged by limitations in biological sourcing. The recent progress in synthetic biology combined to the increasing access to extensive transcriptomics and genomics data now provide new alternatives to produce these molecules by transferring their whole biosynthetic pathway in heterologous production platforms such as yeasts or bacteria. While the generation of high titer producing strains remains per se an arduous field of investigation, elucidation of the biosynthetic pathways as well as characterization of their complex subcellular organization are essential prequels to the efficient development of such bioengineering approaches. Using examples from plants and yeasts as a framework, we describe potent methods to rationalize the study of partially characterized pathways, including the basics of computational applications to identify candidate genes in transcriptomics data and the validation of their function by an improved procedure of virus-induced gene silencing mediated by direct DNA transfer to get around possible resistance to Agrobacterium-delivery of viral vectors. To identify potential alterations of biosynthetic fluxes resulting from enzyme mislocalizations in reconstituted pathways, we also detail protocols aiming at characterizing subcellular localizations of protein in plant cells by expression of fluorescent protein fusions through biolistic-mediated transient transformation, and localization of transferred enzymes in yeast using similar fluorescence procedures. Albeit initially developed for the Madagascar periwinkle, these methods may be applied to other plant species or organisms in order to establish synthetic biology platform. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
BIOLISTIC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CANDIDATE GENE IDENTIFICATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CATHARANTHUS ROSEUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FLUORESCENT PROTEIN FUSIONS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SPECIALIZED METABOLISM |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SUBCELLULAR LOCALIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SYNTHETIC BIOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
VIRUS-INDUCED GENE SILENCING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
YEAST BIOENGINEERING |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Foureau, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Carqueijeiro, I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dugé De Bernonville, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Melin, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lafontaine, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Besseau, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lanoue, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lanoue, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Oudin, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Glévarec, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Clastre, M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
St-Pierre, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Giglioli-Guivarc'H, N. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Courdavault, V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1elKIeFesId-K8YA9I96j0qSCV8I_nFZQ/view?usp=drivesdk">https://drive.google.com/file/d/1elKIeFesId-K8YA9I96j0qSCV8I_nFZQ/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
