MARC details
| 000 -LEADER |
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| fixed length control field |
02166nam a2200385Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160146.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16160 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Molecular Cell, 60(3), p.385-397, 2015 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and Cpf1, are known. We describe here three distinct Class 2 CRISPR-Cas systems. The effectors of two of the identified systems, C2c1 and C2c3, contain RuvC-like endonuclease domains distantly related to Cpf1. The third system, C2c2, contains an effector with two predicted HEPN RNase domains. Whereas production of mature CRISPR RNA (crRNA)by C2c1 depends on tracrRNA, C2c2 crRNA maturation is tracrRNA independent. We found that C2c1 systems can mediate DNA interference in a 5'-PAM-dependent fashion analogous to Cpf1. However, unlike Cpf1, which is a single-RNA-guided nuclease, C2c1 depends on both crRNA and tracrRNA for DNA cleavage. Finally, comparative analysis indicates that Class 2 CRISPR-Cas systems evolved on multiple occasions through recombination of Class 1 adaptation modules with effector proteins acquired from distinct mobile elements. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CASPASE 9 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CPF1 PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CRISPR ASSOCIATED PROTEIN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ENDONUCLEASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RIBONUCLEASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
RNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
UNCLASSIFIED DRUG |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Shmakov, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Abudayyeh, O.O. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Makarova, K.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wolf, Y.I. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Gootenberg, J.S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Semenova, E. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Minakhin, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Minakhin, L. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Konermann, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Severinov, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zhang, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Koonin, E.V. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1o0EEqZDhzJD9Wy6IgI6oV3bR9QWtJBNQ/view?usp=drivesdk">https://drive.google.com/file/d/1o0EEqZDhzJD9Wy6IgI6oV3bR9QWtJBNQ/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
