Direct somatic embryogenesis and organogenesis in cultured immature zygotic embryos of Pisum sativum L. (Record no. 50353)

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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16179
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245 10 - TITLE STATEMENT
Title Direct somatic embryogenesis and organogenesis in cultured immature zygotic embryos of Pisum sativum L.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 137(1), p.102-109, 1990
520 3# - SUMMARY, ETC.
Summary, etc. Nine cultivars of Pisum sativum L. were screened for their ability to develop somatic embryos and buds from immature zygotic embryos cultured in vitro. In certain cultivars, such as «Bonnaire» and «Davina», high frequency regeneration was achieved via direct somatic embryogenesis or organogenesis, depending on the composition of the medium. Embryogenesis was achieved when zygotic embryos were plated in darkness for 4-5 weeks on MS medium (Murashige and Skoog, 1962)containing 43 µM NAA, thiamine hydrochloride (15 µM), nicotinic acid (40 µM)and arginine (60 µM). However, the percentage of somatic embryos obtained depended on the cultivar used. These embryos developed into plantlets when cultured on MS + 0.01 M KNO 3 supplemented with 15 µM IBA and 2.2 µM BAP. Direct organogenesis was obtained when MS containing 16 µM NAA, 13.3 µM BAP and 0.2 µM TIBA was used. Regenerated shoots were rooted on the same medium, but without TIBA. Following transfer to soil, the plants developed normally and set seed. Histological studies revealed that proliferating somatic embryos or buds originated directly from the cotyledons, without an intermediate callus stage. The cultivars «Bemol» and «Mini» were among the lines that could not be regenerated
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PISUM SATIVUM L.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element IMMATURE ZYGOTIC EMBRYO
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ORGANOGENESTS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE CULTURE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Tétu, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sangwan, R.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sangwan-Norreel, B.S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1-WxdYhEE-scRGY6Wsfl5KrpWFefL-X-j/view?usp=drivesdk">https://drive.google.com/file/d/1-WxdYhEE-scRGY6Wsfl5KrpWFefL-X-j/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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