The Development of ELISA and IRA for Cytokinin Estimation and their Application to a Study of Lunar Periodicity in Ecklonia maxima (Osbeck)Papenf. (Record no. 50396)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16222
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245 10 - TITLE STATEMENT
Title The Development of ELISA and IRA for Cytokinin Estimation and their Application to a Study of Lunar Periodicity in Ecklonia maxima (Osbeck)Papenf.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 122(5), p.455-466, 1986
520 3# - SUMMARY, ETC.
Summary, etc. Changes in the cytokinin-like activity in the seaweed Ecklonia maxima during a lunar cycle was determined. Bioassays were performed on lamina, stipe and holdfast material individually. For a more detailed investigation on the cytokinins in the stipe, which is the commercially important component, several immunoassays were developed. An enzyme-linked immunosorbent assay was developed for isopentenyladenosine (IPA), but because of low precision this assay system was not suitable for isopentenyladenine analysis. Sensitive radioimmunoassays were developed for zeatin (Z)and ribosylzeatin (ZR). Bioassay results indicated that cytokinin-like activity in the stipe, lamina and holdfast material followed similar trends, which suggested a close link to lunar periodicity. The levels of activity reached a maximum at approximately full moon and last quarter. Immunoassay of the stipe indicated the presence of Z, ZR and IPA. Their fluctuations in concentration during the lunar cycle were similar to that obtained by bioassay, with IPA being detected in the highest concentrations.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ECKLONIA MAXIMA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LUNAR CYCLE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOASSAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element RADIOIMMUNOASSAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ENZYME IMMUNOASSAY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CYTOKININS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hofman, P.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Featonby-Smith, B.C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vanstaden, J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1TDg5XdV94skS-sV-C5ytvGfCngGbTcra/view?usp=drivesdk">https://drive.google.com/file/d/1TDg5XdV94skS-sV-C5ytvGfCngGbTcra/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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