Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter (Record no. 50476)
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| fixed length control field | 02160nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160149.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16302 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Gene, 32(3), p.263-274, 1984 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The promoter region from the cloned glyceraldehyde-3-phosphate dehydrogenase (GPD)gene of Saccharomyces cerevisiae (Musti et al., 1983)has been characterized. A 653-bp TaqI restriction fragment with a 3 ' border 24 bp upstream from the ATG initiation codon was isolated and demonstrated to contain all sequences necessary for promoter function in vivo. This DNA segment was converted to a portable promoter by cloning it into M13mp9, and the entire nucleotide sequence of the portable promoter was determined. Two generalized yeast expression vectors have been constructed utilizing the GPD portable promoter. The expression vectors include the yeast 2/gm origin of replication and amplification functions, such that the plasmids are maintained at high copy number in cir° yeast hosts. These vectors direct synthesis of a consensus ?-interferon(IFN-?Con1)as 1 percent of total cell protein. Hepatitis B surface antigen (HBsAg)was also expressed from these vectors. The 5 ' end of the HBsAg gene was replaced with a synthetic DNA segment which restored the deleted GPD untranslated leader and utilized optimal yeast codons for the first 30 amino acids. The partially synthetic gene resulted in a 10- to 15-fold increased expression level from GPD vectors yielding HBsAg polypeptide as 2-4 percent of total cell protein. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | ALPHA INTERFERON |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | HEPATITIS B SURFACE ANTIGEN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | RECOMBINANT DNA |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bitter, G.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Egan, K.M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1yG1B_U0VZMwmz3v_u5-CKlajGjzoGkO5/view?usp=drivesdk">https://drive.google.com/file/d/1yG1B_U0VZMwmz3v_u5-CKlajGjzoGkO5/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16302 | 25.06.2025 | 25.06.2025 | Documentos solicitados |