MARC details
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02210nam a2200301Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20250625160150.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16391 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Force generation of organelle transport measured in vivo by an infrared laser trap |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Nature, 348(6299), p.346-348, 1990 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
ORGANELLE transport along microtubules is believed to be mediated by organelle-associated force-generating molecules1. Two classes of microtubule-based organelle motors have been identified: kinesin2-7 and cytoplasmic dynein8-12. To correlate the mechanochemical basis of force generation with the in vivo behaviour of organelles, it is important to quantify the force needed to propel an organelle along microtubules and to determine the force generated by a single motor molecule. Measurements of force generation are possible under selected conditions in vitro (for example, see refs 13 and 14), but are much more difficult using intact or reactivated cells. Here we combine a useful model system for the study of organelle transport, the giant amoeba Reticulomyxa 15, with a novel technique for the non-invasive manipulation of and force application to subcellular components, which is based on a gradient-force optical trap, also referred to as 'optical tweezers'16-19. We demonstrate the feasibility of using controlled manipulation of actively translocating organelles to measure direct force. We have determined the force driving a single organelle along microtubules, allowing us to estimate the force generated by a single motor to be 2.6 × 10-7 dynes. © 1990 Nature Publishing Group. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AMOEBA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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ARTICLE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CELL ORGANELLE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
INTRACELLULAR TRANSPORT |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MICROTUBULE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NONHUMAN |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PRIORITY JOURNAL |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ashkin, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Schütze, K. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dziedzic, J.M. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Euteneuer, U. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Schliwa, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1qf_mWsgXfg3POr9LK6nAF-kJ-p7tq_rK/view?usp=drivesdk">https://drive.google.com/file/d/1qf_mWsgXfg3POr9LK6nAF-kJ-p7tq_rK/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
