Purification of Proteins and the Disruption of Microbial Cells (Record no. 50591)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160151.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16417
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245 10 - TITLE STATEMENT
Title Purification of Proteins and the Disruption of Microbial Cells
490 0# - SERIES STATEMENT
Volume/sequential designation Biotechnology Progress, 3(1), p.31-42, 1987
520 3# - SUMMARY, ETC.
Summary, etc. Intracellular proteins with catalytic or biological activity are of growing importance for developments in enzyme technology, as well as for the production of mammalian proteins by recombinant-DNA technology. The release of these proteins from microorganisms is an important unit operation, as it is the first step in their isolation. Gram-scale disruption of microorganisms can be performed by a variety of established methods based on chemical, enzymatic, physical, or mechanical principles. For the large scale disruption of microorganisms, mechanical disintegrators, such as high-speed agitator bead mills or high-pressure industrial homogenizers, are commonly employed. Both types of equipment were designed originally for other tasks; in the paint industry or in the milk industry, respectively. Therefore, it appeared necessary and possible to improve design and performance for the application in cell disintegration. The goal is a uniform exposure of the microbial cells and a minimal exposure of solubilized protein to high shear forces, in order to obtain high yields and to avoid the generation of too small cell wall fragments, which are difficult to separate. Both types of machines have been investigated for the disintegration of different microbial cells and the influence of the operating parameters analyzed on protein solubilization and enzyme yield. We will summarize the state of the art and discuss new data to illustrate trends in process development. Copyright © 1987 American Institute of Chemical Engineers (AIChE)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOLOGICAL MATERIALS - CELLS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element BIOTECHNOLOGY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRUSHING AND GRINDING
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICROORGANISMS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kula, M.-R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Schütte, H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1jc-a-sGSY6gXuoSNhAygTZdNwxOXE6w-/view?usp=drivesdk">https://drive.google.com/file/d/1jc-a-sGSY6gXuoSNhAygTZdNwxOXE6w-/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16417 25.06.2025 25.06.2025 Documentos solicitados