MARC details
| 000 -LEADER |
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| fixed length control field |
02872nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160151.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16427 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Optimization and partial characterization of intracellular anticandidal protein from Aspergillus giganteus MTCC 8408 using taguchi DOE |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Bioengineered, 14 January, p.1-13, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
A new intracellular antifungal protein (afp)production with average molecular weight 24.3 kDa and yield of 0.65 ± 0.1 mg/gram dry cell weight (gdcw)of mycelia in submerged fermentation of Aspergillusgiganteus MTCC 8408 was optimized. Taguchi's DOE (design of experiment)L27 orthogonal array (OA)was constructed using Qualitek-4 software with 8 most influensive factors namely, culture pH, temperature, slant age, inoculum volume, agitation and KH2PO4. Scanning electron microscopy (SEM)was used to correlate the effect of selected factors on fungal cell morphology and afp production. The crude protein purification was accomplished using pure ammonium sulfate fractionation followed by carboxymethyl cellulose (CMC)ion-exchange chromatography and sephadex G-100 gel filtration. The average molecular mass of the purified protein was figured by silver stained SDS (sodium dodecylsulphate)-PAGE (poly-acryl amide gel electrophoresis). In vitro antifungal susceptibility assay was profiled against Candida albicans NCIM 3471 and minimum inhibitory concentrations (MICs)were in the range 3 to 4 µg/ml. Characterization of protein was observed with FTIR (Fourier transform infrared spectroscopy)analysis. The optimal production condition for crude afp was obtained as follows: soluble starch: 20 g/l; Corn steep liquor (CSL, 2 per cent)+ proteose peptone (PP, 1 per cent): 30 g/l; pH: 5.8; temperature: 25°C; slant age: 3 d; inoculum size: 5 per cent (v/v); agitation: 180 rpm; KH2PO4: 0.1 g/l. The validation experiments using optimized conditions confirmed an improvement in afp production by 59.4 per cent against the expected enhancement of afp production by 61.22 per cent. The present statistical optimization study revealed an opportunity to promote economical design at the industrial level for future scale up of effective antifungal agent against opportunistic oral and vaginal infection. © 2017 Taylor & Francis |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTIFUNGAL PROTEIN (AFP) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ASPERGILLUS GIGANTEUS MTCC: 8408 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CANDIDA ALBICANS NCIM 3471 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHARACTERIZATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MINIMUM INHIBITORY CONCENTRATION (MIC) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TAGUCHI (DOE)L27 ORTHOGONAL ARRAY (OA) |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dutta, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Das, M.D. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1Kmz8rmcHGoyTzxE8flz9il6VqlmUDN8S/view?usp=drivesdk">https://drive.google.com/file/d/1Kmz8rmcHGoyTzxE8flz9il6VqlmUDN8S/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
