MARC details
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02325nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160153.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16538 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Nature Protocols, 9(2), p.337-361, 2014 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Cytosine methylation within RNA is common, but its full scope and functions are poorly understood, as the RNA targets of most mammalian cytosine RNA methyltransferases (m 5 C-RMTs)remain uncharacterized. To enable their characterization, we developed a mechanism-based method for transcriptome-wide m 5 C-RMT target profiling. All characterized mammalian m 5 C-RMTs form a reversible covalent intermediate with their cytosine substrate-a covalent linkage that is trapped when conducted on the cytosine analog 5-azacytidine (5-aza-C). We used this property to develop Aza-immunoprecipitation (Aza-IP), a methodology to form stable m 5 C-RMT-RNA linkages in cell culture, followed by IP and high-throughput sequencing, to identify direct RNA substrates of m 5 C-RMTs. Remarkably, a cytosine-to-guanine (C?G)transversion occurs specifically at target cytosines, allowing the simultaneous identification of the precise target cytosine within each RNA. Thus, Aza-IP reports only direct RNA substrates and the C?G transversion provides an important criterion for target cytosine identification, which is not available in alternative approaches. Here we present a step-by-step protocol for Aza-IP and downstream analysis, designed to reveal identification of substrate RNAs and precise cytosine targets of m 5 C-RMTs. The entire protocol takes 40-50 d to complete. © 2014 Nature America, Inc. All rights reserved. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
AZACITIDINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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CYTOSINE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENE EXPRESSION PROFILING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
HUMANS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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IMMUNOPRECIPITATION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRNA METHYLTRANSFERASES |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Khoddami, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Cairns, B.R. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/13F9rvYwG6vF8-8UZqI_Y0ma5isnDV4Ok/view?usp=drivesdk">https://drive.google.com/file/d/13F9rvYwG6vF8-8UZqI_Y0ma5isnDV4Ok/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
