A protocol for in vivo detection of reactive oxygen species (Record no. 50712)
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| fixed length control field | 01898nam a2200205Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160153.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16542 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | A protocol for in vivo detection of reactive oxygen species |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Protocol Exchange Community Contributed, p.doi:10.1038/nprot.2008.23, 2008 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | NOTA: EL DOCUMENTO SOLO SE PUEDE CONSULTAR EN LINEA: https://www.nature.com/protocolexchange/protocols/414#/main 2',7'-dichlorofluorescein (H2DCF)and Dihydroethidium (DHE), have been used extensively in tissue culture experiments to evaluate reactive oxygen species (ROS)production. However, it will be more advantageous to be able to detect real time ROS production in live tissues, especially in Drosophila where the extensive genetic tools available make it possible to compare the phenotype of mutant tissue juxtaposed to its wild-type neighbor. Here, we describe a protocol for imaging ROS production in Drosophila using either H2DCF or DHE. We highlight the specific advantage posed by monitoring ROS production in vivo by comparing the phenotype of cells mutant for genes encoding mitochondrial proteins with their wild-type neighbors. We also show from staining of the germarium that this technique is capable of detecting different levels of ROS production among cells within the same tissue. The whole protocol, from dissection to capturing of images by confocal microscopy can be completed within 2 to 3 hours; and it can be adapted for use in virtually any Drosophila tissue. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | OXYGEN SPECIES |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Owusu-Ansah, Edward |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yavari, Amir |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Banerjee, Utpal |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1CHYMj0GSI7sEU1z-CunhUZvCBSnw6W8m/view?usp=drivesdk">https://drive.google.com/file/d/1CHYMj0GSI7sEU1z-CunhUZvCBSnw6W8m/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16542 | 25.06.2025 | 25.06.2025 | Documentos solicitados |