The detection of tomato spotted wilt virus using the polymerase chain reaction (Record no. 50757)

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control field MX-MdCICY
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control field 20250625160154.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16587
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Title The detection of tomato spotted wilt virus using the polymerase chain reaction
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Volume/sequential designation J Virol Methods, 46(3), p.303-11, 1994
520 3# - SUMMARY, ETC.
Summary, etc. A polymerase chain reaction (PCR)based assay was used to detected a number of UK tomato spotted wilt virus (TSWV)isolates in total RNA extractions made from infected plant material. Extracts were reverse transcribed and the resultant cDNA amplified by PCR, using oligonucleotide primers specific for a 276 base pair fragment of the L RNA segment. Assay products were electrophoresed on agarose gels and visualised by ethidium bromide staining. The viral origin of the product produced was confirmed by sequencing, with the data obtained having very high homology with previously published L RNA sequence data. The specificity and sensitivity of the RT-PCR assay, in comparison with existing tests, is discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element VIRUS
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Topical term or geographic name entry element POLYMERASE CHAIN REACTION
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Personal name Mumford Ra
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Personal name Barker I
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Personal name Wood Kr.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1vQV2OhQZejomIqq_nOMYv9rldkvZ0D8M/view?usp=drivesdk">https://drive.google.com/file/d/1vQV2OhQZejomIqq_nOMYv9rldkvZ0D8M/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16587 25.06.2025 25.06.2025 Documentos solicitados