An improved method for the measurement of cell wall invertase activity in sugarcane tissue (Record no. 50794)

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control field MX-MdCICY
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control field 20250625160155.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16624
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245 10 - TITLE STATEMENT
Title An improved method for the measurement of cell wall invertase activity in sugarcane tissue
490 0# - SERIES STATEMENT
Volume/sequential designation Australian Journal of Plant Physiology, 28(4), p.323-328, 2001
520 3# - SUMMARY, ETC.
Summary, etc. Extraction and assay methods were developed for the determination of both soluble and cell wall invertase activity in sugarcane (Saccharum sp.)from minimal (0.5 g)tissue. Cell wall invertase (CWI)was measured using a pellet mix procedure and the pH optima ranged between pH 3.2 and 3.6. The pH optima for the soluble invertases were 4.5 and 7.3 for soluble acid (SAI)and neutral (NI)invertase, respectively. At low pH, acid hydrolysis of sucrose was observed and its spurious effect on measured enzyme activity was removed by the inclusion of additional controls run in parallel, which lacked crude plant extract. Invertase activity was examined in sugarcane tissues of varying ages. In leaves and stem, the SAI activity was greatly reduced in mature tissue extracts. Similarly, the CWI activity was reduced in older leaves. In contrast, a less marked drop in NI activity was observed in extracts from old leaves and activity from stem extracts remained constant irrespective of tissue age. The role of SAI has been linked to growth and differentiation and these observations suggest that CWI may also be intrinsically involved in these processes. NI appears to have a housekeeping role in maintaining hexose concentrations within the cytosol.
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Topical term or geographic name entry element ACIDS
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Topical term or geographic name entry element BIOASSAY
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Topical term or geographic name entry element CELLS
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Topical term or geographic name entry element ENZYME KINETICS
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Topical term or geographic name entry element GROWTH KINETICS
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Topical term or geographic name entry element HYDROLYSIS
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Topical term or geographic name entry element PH
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SUGAR (SUCROSE)
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Albertson, P.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Peters, K.F.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Grof, C.P.L.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1UjT1e28l5M_LW17JDXtjjV4r5mUutlRn/view?usp=drivesdk">https://drive.google.com/file/d/1UjT1e28l5M_LW17JDXtjjV4r5mUutlRn/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16624 25.06.2025 25.06.2025 Documentos solicitados