MARC details
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03527nam a2200373Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160155.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16638 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
The Microphenotron: A robotic miniaturized plant phenotyping platform with diverse applications in chemical biology |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Plant Methods, 13(1), p.Article number 10, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Background: Chemical genetics provides a powerful alternative to conventional genetics for understanding gene function. However, its application to plants has been limited by the lack of a technology that allows detailed phenotyping of whole-seedling development in the context of a high-throughput chemical screen. We have therefore sought to develop an automated micro-phenotyping platform that would allow both root and shoot development to be monitored under conditions where the phenotypic effects of large numbers of small molecules can be assessed. Results: The 'Microphenotron' platform uses 96-well microtitre plates to deliver chemical treatments to seedlings of Arabidopsis thaliana L. and is based around four components: (a)the 'Phytostrip', a novel seedling growth device that enables chemical treatments to be combined with the automated capture of images of developing roots and shoots; (b)an illuminated robotic platform that uses a commercially available robotic manipulator to capture images of developing shoots and roots; (c)software to control the sequence of robotic movements and integrate these with the image capture process; (d)purpose-made image analysis software for automated extraction of quantitative phenotypic data. Imaging of each plate (representing 80 separate assays)takes 4 min and can easily be performed daily for time-course studies. As currently configured, the Microphenotron has a capacity of 54 microtitre plates in a growth room footprint of 2.1 m2, giving a potential throughput of up to 4320 chemical treatments in a typical 10 days experiment. The Microphenotron has been validated by using it to screen a collection of 800 natural compounds for qualitative effects on root development and to perform a quantitative analysis of the effects of a range of concentrations of nitrate and ammonium on seedling development. Conclusions: The Microphenotron is an automated screening platform that for the first time is able to combine large numbers of individual chemical treatments with a detailed analysis of whole-seedling development, and particularly root system development. The Microphenotron should provide a powerful new tool for chemical genetics and for wider chemical biology applications, including the development of natural and synthetic chemical products for improved agricultural sustainability. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ARABIDOPSIS THALIANA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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AUTOMATED |
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BIOSTIMULANTS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CHEMICAL BIOLOGY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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CHEMICAL GENETICS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ERAGROSTIS TEF |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PLANT PHENOTYPING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ROBOTIC |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ROOT SYSTEM ARCHITECTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SHOOT DEVELOPMENT |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Burrell, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Fozard, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Holroyd, G.H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
French, A.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Pound, M.P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bigley, C.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
James Taylor, C. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
James Taylor, C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1q4l0LPWl2-JBrqnDG1PMxdUd4ZgtwjgL/view?usp=drivesdk">https://drive.google.com/file/d/1q4l0LPWl2-JBrqnDG1PMxdUd4ZgtwjgL/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
