An Improved Transient Expression System Using Arabidopsis Protoplasts (Record no. 50816)

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control field MX-MdCICY
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control field 20250625160155.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16646
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Title An Improved Transient Expression System Using Arabidopsis Protoplasts
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Volume/sequential designation Current Protocols in Plant Biology, 1, p.285-291, 2016
520 3# - SUMMARY, ETC.
Summary, etc. Transient gene expression in protoplasts provides a powerful tool to study protein expression, protein localization, protein-protein association, and gene expression regulation, etc. There are several methods including electroporation, which have been reported to introduce DNA into protoplasts. However, one of the best methods used is polyethylene glycol (PEG)-mediated transfection. Here, we describe an improved PEG-mediated transformation method including preparation of protoplasts, PEG-mediated transformation, and, by way of example, expression of the AtLYK5 gene (AT2G33580)in protoplasts. The protoplast transient expression system provides unique capabilities to support cell-based experiments involved in plant biochemistry and physiology.
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Topical term or geographic name entry element ARABIDOPSIS
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Topical term or geographic name entry element PROTOPLAST
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Topical term or geographic name entry element TRANSIENT EXPRESSION
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Personal name Cao, Yangrong
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Li, Hong
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Pham, An Q.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1-cPPJBx1SUymUxUSJx9gTm6inL7kGyjc/view?usp=drivesdk">https://drive.google.com/file/d/1-cPPJBx1SUymUxUSJx9gTm6inL7kGyjc/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16646 25.06.2025 25.06.2025 Documentos solicitados