MARC details
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02530nam a2200265Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20250625160155.0 |
| 040 ## - CATALOGING SOURCE |
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CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16647 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
A transient gene expression system in Populus euphratica Oliv. protoplasts prepared from suspension cultured cells |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Acta Physiologiae Plantarum, 37(8), p.Article number 160, 8p, 2015 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Populus euphratica Oliv., a species of the model woody plant genus Populus, is well known for its tolerance to salinity stress, the underlying mechanism of which is a research hotspot. Transient expression of fluorescent fusion proteins is commonly used for rapid assessment of gene functions and interactions, and thus would be useful to study the genes involved in salt tolerance in this species. Our transient gene expression protocol for P. euphratica included a simple protoplast preparation and transformation procedure from suspension cultured cells. The highest protoplast yield (8 × 107 g?1 fresh weight)with high viability (above 90 percent)was obtained using an optimized enzyme mix of 4 percent (w/v)cellulase R10, 0.5 percent (w/v)pectinase, and 0.2 percent (w/v)hemicellulase. Factors affecting protoplast transformation efficiency were also optimized: 20 ?g plasmid DNA versus 105 protoplasts, and a transformation time of 20 min using PEG, which resulted in a transformation efficiency greater than 50 percent. A pair of known markers was simultaneously and correctly expressed in the same P. euphratica protoplasts by co-transformation. The isolation and transformation protocol took 5 h, and results could be obtained within 24 h. This protoplast transient expression system is suitable for studying gene expression, protein localization, and protein-protein interactions in woody plants. In addition, it would be particularly useful for studying the signaling pathway involved in the salt tolerance of P. euphratica in a homologous system, which may not even be possible using protoplasts prepared from other species. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
POPULUS EUPHRATICA OLIV |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTOPLAST |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SUSPENSION CULTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
TRANSIENT GENE EXPRESSION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Guo, Y. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Song, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhao, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lv, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Lu, M. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1x6pAPjbZK8CbzpyqhkBt32CxE-oPqCKD/view?usp=drivesdk">https://drive.google.com/file/d/1x6pAPjbZK8CbzpyqhkBt32CxE-oPqCKD/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
