MARC details
| 000 -LEADER |
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| fixed length control field |
01909nam a2200289Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160155.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16662 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1 |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Molecular Cell, 67(4), p.633-645.e3, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The RNA-guided Cpf1 (also known as Cas12a)nuclease associates with a CRISPR RNA (crRNA)and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1)and Lachnospiraceae bacterium (LbCpf1)have been harnessed for eukaryotic genome editing. Cpf1 requires a specific nucleotide sequence, called a protospacer adjacent motif (PAM), for target recognition. Besides the canonical TTTV PAM, Cpf1 recognizes suboptimal C-containing PAMs. Here, we report four crystal structures of LbCpf1 in complex with the crRNA and its target DNA containing either TTTA, TCTA, TCCA, or CCCA as the PAM. These structures revealed that, depending on the PAM sequences, LbCpf1 undergoes conformational changes to form altered interactions with the PAM-containing DNA duplexes, thereby achieving the relaxed PAM recognition. Collectively, the present structures advance our mechanistic understanding of the PAM-dependent, crRNA-guided DNA cleavage by the Cpf1 family nucleases. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CAS12A |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CPF1 |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRISPR-CAS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRYSTAL STRUCTURE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PROTOSPACER ADJACENT MOTIF |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yamano, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zetsche, B. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ishitani, R. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Nishimasu, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Nureki, O. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1pgCcAX6VCFBa1Nb6CeLQ9wI6sElN_7bs/view?usp=drivesdk">https://drive.google.com/file/d/1pgCcAX6VCFBa1Nb6CeLQ9wI6sElN_7bs/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
