Growth, Cytology and Flow Cytometry of Embryogenic Cell Suspension Cultures of Panicum maximum Jacq. and Pennisetum purpureum Schum. (Record no. 50838)

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fixed length control field 02212nam a2200397Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160155.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16668
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Growth, Cytology and Flow Cytometry of Embryogenic Cell Suspension Cultures of Panicum maximum Jacq. and Pennisetum purpureum Schum.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 123(3), p.211-227, 1986
520 3# - SUMMARY, ETC.
Summary, etc. Embryogenic cell suspensions of Panicum maximum (4 year old)and Pennisetum purpureum (3 year old)continue rapid growth upon subculture without a marked lag phase. They have cell doubling times of 27 h and 32 h, respectively, during the exponential phase of growth. Maximum cell numbers of 2 × 10? cells · ml-1 are reached on about the eighth day of subculture in both species. Thereafter, cell lysis and death occur progressively. By day 16, 90 percent of P. purpureum cells appear non-viable. Flow cytometry of the cell suspensions showed that at any given time during the 8-9 day culture period, a majority of the cells are in G1 phase of the cell cycle, with substantial numbers cycling through the S and G2 phases. No marked change in this distribution pattern was seen in the first 8-9 days of growth during which the cultures did not reach stationary phase. Flow cytometry, microspectrophotometry and chromosome counts showed the cultures comprised predominantly ofnormal diploid cells.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 2,4-D
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 2,4-DICHLOROPHENOXYACETIC ACID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL CYCLE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL SUSPENSION CULTURE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CEREALS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DRY WEIGHT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DW
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FLOW CYTOMETRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FRESH WEIGHT
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FW
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GRASSES
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element MICROSPECTROPHOTOMETRY
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PACKED CELL VOLUMEN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PANICUM MÁXIMUM
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCV
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PCV
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element TISSUE CULTURE
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Karlsson, S.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Vasil, I.K.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1XsHxI796IuBZUMdFkeRZk8l94sdZbd-s/view?usp=drivesdk">https://drive.google.com/file/d/1XsHxI796IuBZUMdFkeRZk8l94sdZbd-s/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16668 25.06.2025 25.06.2025 Documentos solicitados