Modeling linear and variable growth in phosphate limited suspension cultures of Opium poppy (Record no. 50849)

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control field 20250625160156.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16679
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245 10 - TITLE STATEMENT
Title Modeling linear and variable growth in phosphate limited suspension cultures of Opium poppy
490 0# - SERIES STATEMENT
Volume/sequential designation Biotechnology and BioEngineering, 38(4), p.371-379, 1991
520 3# - SUMMARY, ETC.
Summary, etc. In examining the growth kinetics of cell suspensions of opium poppy (Papaver somniferum), the increase in biomass with time was observed to be linear over the entire batch growth period of up to 20 days. Although batch growth profiles were reproducible utilizing the same inoculum, growth rates varied tremendously when experiments were inoculated with cells from different flasks. Both of these phenomena are difficult to explain with conventional batch growth models. In a series of a experiments, phosphate was determined to be the growth-rate-limiting substrate. By expressing growth rate in terms of the intracellular reserves of phosphorus, a growth model which expresses kinetics in terms of the intracellular phosphorus contents of the cells is shown to predict both linear growth character and inoculum dependent variability in growth. The stationary phase phosphate content of seven plant suspension cultures of different plant species was found to be comparable to phosphorus levels of phosphate-starved poppy cells, which suggests that phosphate limitation may be common for plant tissue culture. The applicability of this model to other biological systems which display similar batch growth patterns when subjected to inorganic nutrient deprivation is discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CELL CULTURE--GROWTH KINETICS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element METABOLISM--MATHEMATICAL MODELS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHOSPHATES--EFFECTS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Curtis, W.R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Hasegawa, P.M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Emery, A.H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16Ux3GDGcBoC98bMIkyKTcrEMYce0he1k/view?usp=drivesdk">https://drive.google.com/file/d/16Ux3GDGcBoC98bMIkyKTcrEMYce0he1k/view?usp=drivesdk</a>
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16679 25.06.2025 25.06.2025 Documentos solicitados