A Broad-Spectrum Inhibitor of CRISPR-Cas9 (Record no. 50858)

MARC details
000 -LEADER
fixed length control field 02102nam a2200397Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160156.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16688
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245 10 - TITLE STATEMENT
Title A Broad-Spectrum Inhibitor of CRISPR-Cas9
490 0# - SERIES STATEMENT
Volume/sequential designation Cell, 170(6), p.1224-1233.e15, 2017
520 3# - SUMMARY, ETC.
Summary, etc. CRISPR-Cas9 proteins function within bacterial immune systems to target and destroy invasive DNA and have been harnessed as a robust technology for genome editing. Small bacteriophage-encoded anti-CRISPR proteins (Acrs)can inactivate Cas9, providing an efficient off switch for Cas9-based applications. Here, we show that two Acrs, AcrIIC1 and AcrIIC3, inhibit Cas9 by distinct strategies. AcrIIC1 is a broad-spectrum Cas9 inhibitor that prevents DNA cutting by multiple divergent Cas9 orthologs through direct binding to the conserved HNH catalytic domain of Cas9. A crystal structure of an AcrIIC1-Cas9 HNH domain complex shows how AcrIIC1 traps Cas9 in a DNA-bound but catalytically inactive state. By contrast, AcrIIC3 blocks activity of a single Cas9 ortholog and induces Cas9 dimerization while preventing binding to the target DNA. These two orthogonal mechanisms allow for separate control of Cas9 target binding and cleavage and suggest applications to allow DNA binding while preventing DNA cutting by Cas9
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ANTI-CRISPR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CAS9
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRISPR
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRISPR-CAS9
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element GENE EDITING
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Harrington, L.B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Doxzen, K.W.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ma, E.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Liu, J.-J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Knott, G.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Edraki, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Garcia, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Garcia, B.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Chen, J.S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Cofsky, J.C.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kranzusch, P.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sontheimer, E.J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Davidson, A.R.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Maxwell, K.L.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Doudna, J.A.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/16c2_ONNdK6KeyHEGYlbpiOl9aGITkyV8/view?usp=drivesdk">https://drive.google.com/file/d/16c2_ONNdK6KeyHEGYlbpiOl9aGITkyV8/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16688 25.06.2025 25.06.2025 Documentos solicitados