Methylation of DNA in the chloroplasts and amyloplasts of the pea, Pisum sativum (Record no. 50865)

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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160156.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16695
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
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245 10 - TITLE STATEMENT
Title Methylation of DNA in the chloroplasts and amyloplasts of the pea, Pisum sativum
490 0# - SERIES STATEMENT
Volume/sequential designation Plant Science, 78(1), p.33-42, 1991
520 3# - SUMMARY, ETC.
Summary, etc. Digestion with methyl-sensitive and methyl-insensitive isoschisomers of several restriction endonucleases showed that a small but significant amount of cytosine residues of the chloroplast DNA in the pea, Pisum sativum, was methylated. HPLC analysis of component nucleosides indicated that about 0.5 percent of cytosine residues in the chloroplast DNA was methylated at C-5. The methylationwas not related to aging of the plant. Cytosine residues of most of the CC(A/T)GG sites and some of the GATC sites in the chloroplast DNA were partially methylated in three genes examined, rbcL, atpB and psbDC. Most of the cytosine residues at the same sites in the amyloplast DNA were also methylated, while some of the sites were not methylated in the amyloplast DNA. Apparently, these methylation cannot be involved in the regulation of gene expression, and possible roles of the methylation of chloroplast DNA are discussed.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element 5-METHYLCYTOSINE
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element AMYLOPLAST DNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLOROPLAST DNA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA METHYLATION
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PISUM SATIVUM
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Ohta, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Sato, N.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kawano, S.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kuroiwa, T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1wCRHb2Pm-kCYyJLIIXpYIlv7patNDc7S/view?usp=drivesdk">https://drive.google.com/file/d/1wCRHb2Pm-kCYyJLIIXpYIlv7patNDc7S/view?usp=drivesdk</a>
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16695 25.06.2025 25.06.2025 Documentos solicitados