Direct somatic embryogenesis and plant regeneration from leaf explants of Nicotiana tabacum L. (Record no. 50869)

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control field MX-MdCICY
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control field 20250625160156.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-16699
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245 10 - TITLE STATEMENT
Title Direct somatic embryogenesis and plant regeneration from leaf explants of Nicotiana tabacum L.
490 0# - SERIES STATEMENT
Volume/sequential designation Journal of Plant Physiology, 137(3), p.347-357, 1991
520 3# - SUMMARY, ETC.
Summary, etc. irect somatic embryogenesis was induced in leaf disc-cultures of three cultivars of Nicotiana tabacum L. on MS medium supplemented with various concentrations of cytokinins and auxins. The first somatic embryos were formed on the surfaces of discs without callus proliferation, within 10-12 days. Optimal conditions for induction of embryogenic development were found to be a medium containing a combination of BAP (1 mg · L-1)and NAA (0.1 mg · L-1). Development of somatic embryos progressed through typical embryogenic stages, nemely globular, heart-shaped, torpedo-shaped, and cotyledonary stages similar to those of zygotic embryos. Histological analysis strongly indicated that somatic embryos were formed on leaf discs as anatomically independent units with closed radicular ends, and free of vascular connection with the original explant. The sequences of zygotic embryogenesis as well as pollen embryogenesis are presented as the morphological patterns with which to compare the events occurring during somatic embryogenesis from leaf explants. Somatic embryos and microspore-derived embryos are larger in size than zygotic embryos.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element LEAF DISC
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element NICOTIANA TABACUM L.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element POLLEN EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SOMATIC EMBRYOGENESIS
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ZYGOTIC EMBRYOGENESIS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Stolarz, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Macewicz, J.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Lörz, H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1pvV5V7bhE14NHGk4ooiWJ1bKPtmxmXB_/view?usp=drivesdk">https://drive.google.com/file/d/1pvV5V7bhE14NHGk4ooiWJ1bKPtmxmXB_/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-16699 25.06.2025 25.06.2025 Documentos solicitados