MARC details
| 000 -LEADER |
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| fixed length control field |
02405nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160156.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16726 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Molecular Cell, 68(1), p.26-43, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
The past several years have seen an explosion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruitment of a range of DNA and chromatin-modifying enzymes. While homology-directed repair (HDR)coupled with Cas9 nuclease cleavage has been used with great success to repair and re-write genomes, recently developed base-editing systems present a useful orthogonal strategy to engineer nucleotide substitutions. Base editing relies on recruitment of cytidine deaminases to introduce changes (rather than double-stranded breaks and donor templates)and offers potential improvements in efficiency while limiting damage and simplifying the delivery of editing machinery. At the same time, these systems enable novel mutagenesis strategies to introduce sequence diversity for engineering and discovery. Here, we review the different base-editing platforms, including their deaminase recruitment strategies and editing outcomes, and compare them to other CRISPR genome-editing technologies. Additionally, we discuss how these systems have been applied in therapeutic, engineering, and research settings. Lastly, we explore future directions of this emerging technology. CRISPR-mediated base editing relies on recruitment of cytidine deaminases to introduce either precise C>T or diverse C>N changes, while avoiding nuclease-mediated double-strand breaks. Here, we review the different base editing platforms, including their deaminase recruitment strategies and editing outcomes, and discuss their application in therapeutic, engineering, and research settings. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DEAMINASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ENDONUCLEASE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GUIDE RNA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
NUCLEOTIDE |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hess, G.T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Tycko, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yao, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bassik, M.C. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1gYW9ki5KRzUI32iI2lb0LA3XBX09Swq0/view?usp=drivesdk">https://drive.google.com/file/d/1gYW9ki5KRzUI32iI2lb0LA3XBX09Swq0/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
