MARC details
| 000 -LEADER |
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| fixed length control field |
02715nam a2200409Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20250625160157.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-16729 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT |
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| Title |
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex |
| 490 0# - SERIES STATEMENT |
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| Volume/sequential designation |
Cell, 171(2), p.414-426.e12, 2017 |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Prokaryotic cells possess CRISPR-mediated adaptive immune systems that protect them from foreign genetic elements, such as invading viruses. A central element of this immune system is an RNA-guided surveillance complex capable of targeting non-self DNA or RNA for degradation in a sequence- and site-specific manner analogous to RNA interference. Although the complexes display considerable diversity in their composition and architecture, many basic mechanisms underlying target recognition and cleavage are highly conserved. Using cryoelectron microscopy (cryo-EM), we show that the binding of target double-stranded DNA (dsDNA)to a type I-F CRISPR system yersinia (Csy)surveillance complex leads to large quaternary and tertiary structural changes in the complex that are likely necessary in the pathway leading to target dsDNA degradation by a trans-acting helicase-nuclease. Comparison of the structure of the surveillance complex before and after dsDNA binding, or in complex with three virally encoded anti-CRISPR suppressors that inhibit dsDNA binding, reveals mechanistic details underlying target recognition and inhibition. Single-particle cryo-EM structures of a type I-F CRISPR surveillance complex before and after target dsDNA binding, as well as after inhibitor binding, demonstrate unique structural features for dsDNA recognition and a large global elongation of the complex, which may facilitate nuclease recruitment and subsequent target degradation. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ADAPTIVE IMMUNITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ANTI-CRISPR INHIBITORS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CONFORMATIONAL TRANSITIONS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRISPR-CAS CSY COMPLEXES |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRISPR-CAS SYSTEMS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CRYOELECTRON MICROSCOPY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
DNA-PROTEIN INTERACTIONS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
GENE EDITING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
PAM RECOGNITION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Guo, T.W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Bartesaghi, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yang, H. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Falconieri, V. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rao, P. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Merk, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Eng, E.T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Eng, E.T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Fox, T. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Earl, L.A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Patel, D.J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Subramaniam, S. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1m-EDrFiUG5RX-BZqzzLLjkaycVXnrd-_/view?usp=drivesdk">https://drive.google.com/file/d/1m-EDrFiUG5RX-BZqzzLLjkaycVXnrd-_/view?usp=drivesdk</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
