Use of PCR for detection of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka leaf spots in banana and plantain (Record no. 50919)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02096nam a2200193Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625160157.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-16752 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Use of PCR for detection of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka leaf spots in banana and plantain |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Mycological Research, 97(6), p.670-674, 1993 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | There are two Sigatoka leaf spot diseases of banana and plantain, yellow Sigatoka and black Sigatoka. Yellow Sigatoka is caused by Mycosphaerella musicola, and black Sigatoka by M. fijiensis. Because unambiguous diagnosis of these leaf spots is often not possible by symptoms alone, we have used the polymerase chain reaction (PCR)to distinguish between the two species, and to detect the pathogens in DNA from infected leaf tissue. Two 21-base oligonucleotide primers were synthesised from a variable region identified in the nucleotide sequence of the internally transcribed spacer (ITS)1 region of ribosomal DNA (rDNA)of Mycosphaerella fijiensis and M. musicola. These primers (MF137 and MM137 respectively)were used in association with primer R635 (from a conserved sequence of the rDNA). Primers MF137 and R635 produced an amplification product of approximately 1000 bp in size with DNA from M. fijiensis. This product was not formed when DNA of M. musicola, M. minima, or a number of other fungi which are commonly found on banana leaves, was amplified. Primer combination MM117 and R635 produced an amplification product of approximately 1000 bp with DNA from M. musicola. The primers amplified similar sized fragments from the DNA extracted from banana leaf tissue infected with M. fijiensis and M. musicola. Southern hybridization analysis confirmed the fungal origin of these |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | AGRICULTURE |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Johanson, A. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jeger, M.J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1Z0iMi_aAUra9MAcaNxmt8Qq6VyqhpTYg/view?usp=drivesdk">https://drive.google.com/file/d/1Z0iMi_aAUra9MAcaNxmt8Qq6VyqhpTYg/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-16752 | 25.06.2025 | 25.06.2025 | Documentos solicitados |